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KMID : 0545120220320030341
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Journal of Microbiology and Biotechnology
2022 Volume.32 No. 3 p.341 ~ p.347
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Bacterial Community of Galchi-Baechu Kimchi Based on Culture-Dependent and - Independent Investigation and Selection of Starter Candidates
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Kim Tao
Heo So-Jeong
Na Hong-Eun
Lee Ga-Won
Kim Jong-Hoon
Kwak Mi-Sun
Sung Moon-Hee
Jeong Do-Won
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Abstract
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In this study, the bacterial community of galchi-baechu kimchi was determined using culture-based and culture-independent techniques (next generation sequencing:NGS), and showed discrepancies between results. Weissella koreensis and Pediococcus inopinatus were the dominant species according to the NGS results, while Bacillus species and P. inopinatus were dominant in the culture-dependent analysis. To identify safe starter candidates, sixty-five Bacillus strains isolated from galchi-baechu kimchi using culture-dependent methods were evaluated for their antibiotic resistance, presence of toxin genes, and hemolytic activity. Strains were then assessed for salt tolerance and protease and lipase activity. As a result, four strains-B. safensis GN5_10, B. subtilis GN5_19, B. velezensis GN5_25, and B. velezensis GT8-were selected as safe starter candidates for use in fermented foods.
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KEYWORD
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Galchi-baechu kimchi, bacterial community, culture dependent, culture independent, Bacillus, starter
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